Book Review

Microbial Contamination of Plant Tissue Cultures
Volume 4 of Recent Advances in Plant Tissue Culture
by Edwin B. Herman, Editor, Agricell Report

Microbial Contamination of Plant Tissue Cultures is the fourth in a series, based on articles published in Agricell Report, that reports developments in a specific area of plant tissue culture. Unlike most plant tissue culture books, which have a 2-4 year publication delay, the Recent Advances in Plant Tissue Culture series is updated within one month of the book’s publication date. In order to make it easy for the reader to obtain further information on material covered  in  the  book,  a  directory  consisting  of  full  addresses  of  cited  authors  is included. The book also includes a list of recent symposia, a taxonomic index and an index of antimicrobial agents.

Partial Contents: 1. Novel approaches to controlling contamination: Production of antimicrobial agents by plant cell cultures, screening for elicitors that stimulate phytoalexin yield. 2. Plant tissue culture contaminants: Identification, screening, sources, vitropaths, latent contamination. 3. Sterilization of seeds. 4. Disinfestation of explants: Laser radiation, running water treatment, hot water treatment, double disinfestation, sulfite pads, bleach, pre-forcing, internal disinfestation. 5. Sterilization of medium:  Autoclaving and role of activated charcoal, microwaving, diethylpyrocarbonate. 6.Elimination of microbial contaminants:  Density gradient centrifugation, flexible container system, repeated subculture, low free-water medium, acidification, egg white lysozyme. 7. Use of antibiotics: Effectiveness, elimination of latent contamination, phytotoxicity, stimulation of regeneration and growth, use of antibiotic discs. 8. Viral elimination: Petal culture, chemotherapy and combined therapies, multiple lateral shoot technique.  9. Control of nematodes. 10. Non-axenic plant tissue cultures: Non-sterile micropropagation, coculture with bacteria and fungi, microbial promotion of morphogenesis and embryogenesis, microbial stimulation of  secondary metabolite yields. 11. Possibilities and opportunities for non-axenic tissue culture.

Publication: April, 1996,  84 pp., softbound. Price: US$63.00 (US$15.00 additional for air mail delivery outside of U.S., Canada or Mexico). Discount price for 10 or more copies: US$45.00 per copy. The price includes a copy of the October, 1996 issue of Agricell Report, which features highlights of the 1996 Symposium on Bacterial and Bacteria-Like Contaminants of Plant Tissue Cultures, held in Cork Ireland. 

Order from: Agritech Consultants, Inc., PO Box 255, Shrub Oak, NY 10588, U.S.A., Phone/Fax: (914) 528-3469, E-mail:AGRICELL@AOL.COM