In Vitro and ex Vitro Adventitious Root Formation in Asian Jasmine (Trachelospermum asiaticum) II. Physiological Comparions.

by R. C. Apter, Fred T. Davies, Jr., and E. L. McWilliams

In vitro tissue-cultured (TC) and macropropagated (MACRO) 18-day old adventitious roots of Asian jasmine [Trachelospermum asiaticum (Siebold and Zucc.) Nakai] were compared for their ability to absorb and translocate radiolabelled P from a nutrient solution. Samples were taken at 1, 2, 4, 5, 12, and 24 hours after the initial dosage of the nutrient solution with 7.4 x 102 MBq KH232PO4/liter. TC roots were capable of absorbing P, but at significantly lower levels than MACRO roots. Greater P absorption occurred in MACRO roots within the first hour and continued for the duration of the experiment. However, there was no significant difference in the rate of P translocation from roots to shoots between treatments. Root systems formed in vitro survived acclimation and had developed into well-branched root systems after 13 weeks. Reduced P absorption by TC roots did not limit either P translocation or survivability during and after acclimation.

J. Amer. Soc. Hort. Sci. 118(6): 906-909. 1993.