Micropropagation of Crinum 'Ellen Bosanquet' by tri-scales.

by Melanie R. Ulrich, Fred T. Davies, Jr., Yong Cheong Koh, Sharon A. Duray, Jonathan N. Egilla.


Abstract:
Crinum lilies (Crinum spp. L.) are tropical and sub-tropical bulbous plants with excellent potential for southern US landscapes. Unfortunately, the more desirable crinum cultivars are slow and expensive to propaget by traditional offsets. The objective of this research was to develop reliable procedures for multiplying Crinum 'Ellen Bosanquet' by tissue culture. A sterilization procedure for cleaning explants was developed in which bulb chips containing a basal plate were submersed and agitated in 0.525% hypochlorite for 1 h. For shoot formation, tri-scales (three scales attached to a section of the basal plate) were used as the explant source and grown on MS-based media containing five levels of benzyladenine (BA) (0-22.2 micrometers) and five levels of naphtaleneacetic acid (NAA) (0-5.3 micrometers). The greatest shoot formation was obtained from the highest level of BA (22.2 micrometers) without NAA. In a subsequent experiement, explants were cultured for 4 months on media containing 35.5-88.8 micrometers BA, and then transfered to hormone-free media for 3 months. BA at 35.5 micrometers stimulated optimal shoot (8.4) and bulblet (2.8) formation. Plantlets were successfully acclimatized and rooted ex vitro.

Citation:
Scientia Horticulturae 82 (1999) 95-102


PDF:
1999-82-sh