The PC as an Analytical Instrument

INTRODUCTION TO THE WORKSHOP

R. Daniel Lineberger
Department of Horticultural Sciences
Texas A&M University
College Station, TX 77843
Phone 409-845-5278
FAX 409-845-0627
Email dan-lineberger@tamu.edu

EVALUATING SUPERFICIES OF HORTICULTURAL CROPS BY DIGITAL IMAGE ANALYSIS

Steven C. Wiest
Department of Horticulture
Kansas State University
Manhattan, KS 66506-5506
Phone 913-532-1435
FAX 913-532-6949
Email swiest@oz.oznet.ksu.edu

Methods of evaluating the external morphological and color characteristics of horticultural crops (specifically turfgrass canopies and the exterior surface of grape berries) will be discussed, especially in terms of the computerized analysis of digital images. The determination of color by using a hue-saturation-intensity model will be discussed. Classical (e.g., texture) as well as more recent (fractal and wavelet analyses) will also be demonstrated.

THE USE OF INFRARED THERMOGRAPHY IN HORTICULTURE

M. Wisniewski, M. Fuller, and L. Fuchigami. USDA-ARS, Kearneysville, WV; Seale-Hayne College of Agriculture, Plymouth University, Newton Abbot, UK; Department of Horticulture, Oregon State University, Corvallis, OR.
Phone 304-725-3451 ext 320
FAX 304-728-2340
Email MWisniew@afrs.ars.usda.gov

High-resolution infrared thermography is a powerful tool for studying temperature-related phenomena in plants. The accuracy and sensitivity of high end equipment allows this technology to be used in research as well as for educational and industrial applications. To date, we have used this technology largely for studying ice nucleation and propagation in plants. Examples of the use of this technology in our research will be presented. Additionally, examples of both teaching and industrial applications will be presented and discussed.

BIOINFORMATIC TOOLS ON THE WORLD WIDE WEB: PROGRAMS FOR GENE ANALYSIS

Carole L. Bassett
Research Molecular Biologist
USDA, Agricultural Research Service
Appalachian Fruit Research Station
45 Wiltshire Road
Kearneysville, WV 25430
Phone 304 725-3451 ext. 367
FAX 304 728-2340
Email cbassett@afrs.ars.usda.gov

Summary: Identification and determination of the function of genes isolated by standard molecular techniques or in the course of large genome sequencing projects requires rapid and accurate computer methods. Numerous software programs which can be used to identify and characterize an "unknown" gene are freely accessible from the WWW. An overiew of these programs will be presented with a comparison of tools to identify basic promoter elements discussed as an example.

MOVING BEYOND QUALITATIVE DATA ANALYSIS: COMPUTER-ASSISTED QUANTIFICATION OF GELS, BLOTS, AND CHROMATOGRAMS

Mary E. Rumpho and Theodore C. Fox
Department of Horticultural Sciences
Texas A&M University
College Station, TX 77843

Phone 409-862-4260
FAX 409-845-0627
Email m-rumpho@tamu.edu